RNA

Part:BBa_K1559000

Designed by: Xiuqi (Rex) Xia   Group: iGEM14_Toronto   (2014-10-07)

tracr RNA to be used with Cas9

This is base pairs 1 to 543 amplified from Part:BBa_K1218011(biobricked pCas9), which contains the tracrRNA and a region containing a bidirectional promoter. Meant to be used in the assembly of Part:BBa_K1559002

Usage and Biology

This part should be compatible with all Cas9 genes derived from [http://www.addgene.org/42876/ pCas9] (type-II CRISPR locus of Streptococcus pyogenes), such as Part:BBa_K1218011, Part:BBa_K1026001, Part:BBa_K1150000.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None